Freeze - Thaw Injury in Wood Frog Erythrocytes
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منابع مشابه
Real-time measurement of metabolic rate during freezing and thawing of the wood frog, Rana sylvatica: implications for overwinter energy use.
Ectotherms overwintering in temperate ecosystems must survive low temperatures while conserving energy to fuel post-winter reproduction. Freeze-tolerant wood frogs, Rana sylvatica, have an active response to the initiation of ice formation that includes mobilising glucose from glycogen and circulating it around the body to act as a cryoprotectant. We used flow-through respirometry to measure CO...
متن کاملCryoprotection by urea in a terrestrially hibernating frog.
The role of urea as a balancing osmolyte in osmotic adaptation is well known, but this 'waste product' also has myriad other functions in diverse taxa. We report that urea plays an important, previously undocumented role in freezing tolerance of the wood frog (Rana sylvatica), a northern woodland species that hibernates terrestrially in sites where dehydration and freezing may occur. Wood frogs...
متن کاملVertebrate Freeze Tolerance: Molecular Studies of Signal Transduction and Gene Expression
Various amphibians and reptiles have the extraordinary ability to endure the freezing of body fluids while overwintering. In studies with the wood frog, Rana sylvatica, we continue to explore new facets of vertebrate freeze tolerance. New studies have analyzed modifications of wood frog insulin and the controls on protein kinase A and protein phosphatase-1 that regulate glycogen metabolism in o...
متن کاملUrea loading enhances freezing survival and postfreeze recovery in a terrestrially hibernating frog.
We tested the hypothesis that urea, an osmolyte accumulated early in hibernation, functions as a cryoprotectant in the freeze-tolerant wood frog, Rana sylvatica. Relative to saline-treated, normouremic (10 micromol ml(-1)) frogs, individuals rendered hyperuremic (70 micromol ml(-1)) by administration of an aqueous urea solution exhibited significantly higher survival (100% versus 64%) following...
متن کاملGlucose concentration regulates freeze tolerance in the wood frog Rana sylvatica.
In spring, the lowest temperature during freezing that can be survived by wood frogs (Rana sylvatica) from southern Ohio is approximately -3 degrees C. We investigated whether the thermal limit of freeze tolerance in these frogs is regulated by tissue levels of glucose, a putative cryoprotectant that is distributed to tissues during freezing. Frogs receiving exogenous glucose injections prior t...
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تاریخ انتشار 2002